| 別品名 |
HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Serum
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| 適用 |
Western Blot
Dot Blot
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| 免疫動物 |
Rabbit
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| 交差種 |
Human
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| GENE ID |
3569
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| Accession No.(Gene/Protein) |
NP_000591, P05231
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| Gene Symbol |
IL6
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| 形状 |
滅菌済み液状品
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| 参考文献 |
[Pub Med ID]31750769, 19214689
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot using Rockland's Anti IL6 antibody. Protein was resolved on a 4 20% Tris Glycine gel by SDS PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA TBST. Detection occurred using peroxidase conjugated Goat anti Rabbit IgG (p/n 611 103 122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB 070) for 30 min at RT followed by reaction with FemtoMaxTM chemiluminescent substrate. Image was captured using VersaDocTM MP 4000 imaging system (Bio Rad).
Western Blot of Rabbit Anti-IL-6 Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: Human Rec. IL-6/HeLa Whole Cell Lysate [0.05ug/10ug]. Lane 3: Human Rec. IL-6/HeLa Whole Cell Lysate [0.02ug/10ug]. Lane 4: Human Rec. IL-6/HeLa Whole Cell Lysate [0.01ug/10ug]. Lane 5: HeLa Whole Cell Lysate (p/n W09-000-364) [10ug]. Primary Antibody: Anti-IL-6 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP conjugate (p/n 611-103-122) at 1:70,000 for 30mins at RT. Block: BlockOut Buffer (p/n MB-073). Predicted MW: ~23kDa. Observed MW: ~19 Exposure: 2 seconds.
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Western blot using Rockland's Anti IL6 antibody. Protein was resolved on a 4 20% Tris Glycine gel by SDS PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA TBST. Detection occurred using peroxidase conjugated Goat anti Rabbit IgG (p/n 611 103 122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB 070) for 30 min at RT followed by reaction with FemtoMaxTM chemiluminescent substrate. Image was captured using VersaDocTM MP 4000 imaging system (Bio Rad).
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| メーカー |
品番 |
包装 |
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RKL
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109-401-310
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1.0 ML
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※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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